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韩春雨:已有6、7家实验室重复出关键环节

2016-08-12 08:55:54   观察者网

一位不愿具名、熟悉期刊流程的学者向澎湃新闻(www.thepaper.cn)解读“既定流程”的含义时,表达了和韩春雨同样的意思,并表示:“如果没有在学术规范内部做出的质疑的话,学术杂志是没有办法去和作者做质询的。”

对于邮件中摘取的关于公开数据的规定,韩春雨说:“这是他们网站上自带的,原来我投稿的时候就有,是发论文的前提要求。已经做到了,提供过了。”

美国约翰∙霍普金斯大学工学院董事,曾担任Computational Linguistics杂志评委以及许多国际学术会议程序委员会委员、联执主席的吴军则认为,方便同行证实自己的科研成果是科学家的责任,最基本的是在论文中写清实验怎么做,如受论文篇幅限制,那么科学家有义务为同行答疑,私下告诉实验细节。

韩春雨觉得自己是坦诚的,之前有同行因为无法重复,曾列出实验细节来询问如何改进,“真诚写邮件的人,我们一看就知道是认真做实验的,我们一定会给他很好的解释,然后告诉他哪些需要注意。”韩春雨透露,也有实验室跑来当面询问实验细节,还直接待在韩春雨所在的实验楼进行重复实验。

实验细节很关键。各项参数、细节都得一致,稍有出入,就不能称为严格意义上的重复实验。韩春雨指着桌上两只底纹不同的功夫茶杯,反问道:“一样吗?外行人远看觉得是一样。”

当记者询问公开更多数据是否有利于重复实验的开展时,韩春雨的回答是否定的:“公布也没用,那不等于韩寒公布手稿吗?”

但据8月8日《自然》杂志的报道,应Addgene的要求,在8月8日,韩春雨已经向Addgene提交了详细的protocol(实验细节),并希望这可以帮助其他科学家尽快重复出结果。Addgene是一家全球科学家质粒共享的非盈利组织。6月,韩春雨曾将实验所需的质粒上传到Addgene,以便科学家进行NgAgo重复实验。

8月9日,更新版的实验细节已可在Addgene网站下载。韩春雨接受媒体采访时表示,该版本与原先的实验操作“原则上没有本质区别”,是他最近和其他一些研究者交流后,总结了可能被研究者忽视的“特别需要注意的事项”。

据比对,新的实验步骤有几处更改,其中包括“血清品牌由Hyclone变更为Gibco”,“293T细胞贴壁不牢,换液时要小心操作”,“溶解和稀释质粒及gDNA的缓冲液变更为水(pH 8.0)”,“建议在质粒/gDNA共转染的8小时、12小时或24小时后,补转一次gDNA(旧版方法中只提及了‘24小时’)”等内容。

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